Photocopy of sketch in India Ink on a quilt from 1842 (quilt at the Chester County Historical Society, West Chester, Pennsylvania) Photocopy taken by Ned Goode, Apsketch of house in india ink on quilt from 1842 - Primitive Hall, State Route 841 (West Marlborough Township), Clonmell, Chester County, PA Library of Congress Historic Buildings Survey, Historic Engineering Record, Historic Landscapes Survey Photocopy of sketch in India Ink on a quilt from. © 2011 Japanese Dermatological Association. One step staining of Tzanck smear using this diluted ink solution is an inexpensive and a convenient bedside diagnostic tool for the dermatologist. The nuclei of the multinucleated giant cells were clearly stained, and the sample could be immediately used for microscopic examination. Smear of clinical samples from herpes lesions were also stained with 5% ink solution. Blue or black inks or other manufacturers' inks can also be used, but staining of the cultured keratinocytes was less clear. Concentration of ink solution higher or lower than 5% resulted in less contrast. The nuclei of HaCaT were clearly stained showing high contrast with the cytoplasm using 5% Parker-Quink blue-black ink saline solution. Clinical smear samples from herpes lesions were also stained with these solutions without specific pretreatment. A keratinocyte cell line (HaCaT) was cultured on a slide glass and stained with various commercially available blue, blue-black and black inks serially diluted with physiological saline. We report a single step and quick method for staining herpes giant cells in Tzanck smears using routinely available inks and physiological saline. There is a need to develop simple and quick staining methods for bedside diagnosis of this disease.
Giemsa staining for the Tzanck test is time-consuming and laborious. Tzanck test has been recently re-evaluated as a method for the diagnosis of herpes virus infection. Mizutani, Hitoshi Akeda, Tomoko Yamanaka, Kei-Ichi Isoda, Kenichi Gabazza, Esteban C Single step modified ink staining for Tzanck test: quick detection of herpetic giant cells in Tzanck smear.
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